Algae more food. Algae need a light- dark cycle

can be grown in outdoor condition or indoor condition. Sunlight is the most
commonly used source for lighting. Fluorescent lamps provide artificial light
for indoor cultivation. It is important to make the most efficient use of the
artificial light as generation of heat to make the culture too hot. Light
affect to algae photosynthesis. Algae production is high with light intensity
increase due to faster division of algae cells and production of more food.
Algae need a light- dark cycle for productive photosynthesis. The microalgae
cannot use all the supplied light because microalgae convert carbon dioxide in
the air into organic compounds, under visible light condition.Most of algae grow well at temperature from
18-24 0C. Most of algae are die at temperature above 27 0C.
Lower temperature will not cause to kill the algae. But algae growth rate is
reduced. Temperature has positive effect on cell division and photosynthesis
process. Temperature increase trend to enhancement of the enzymatic activities
related to the Calvin cycle. Micro algae growth rate is decrease sharply when
temperature exceeding the optimum level. This is generally caused by heat
stress which can affect the function of enzymatic activities (Ras et al. 2013)

range for most algae species is 7- 9 with the optimum range being 8.2-8.7. Some
are marine species while others are fresh water algae. pH changes may affect
algae growth in many ways. It can change the carbon availability and at extreme
pH level condition may cause direct physiological effect. Normally pH changes
in culture systems caused to correlate with changes in temperature, dis- solved
oxygen, and algae production and distribution. pH may change during the day and
night time. During the day time CO2 absorbed through photosynthesis and that
increase the pH. At the night time CO2 is released through photosynthesis that
caused to decrease the pH level (Zheng 2015).There are two
methods for adjust the experiment pH level. One of pH adjustment method is
providing the extra carbon in the form of CO2 to medium. Second one is  adding diluted acid or base treatment to the
experiment(Chen & Durbin 1994).

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        Salinity variations are in?uenced on the growth and composition
of the microalgae. Algae adaptability to salinity level
also differs.  According to the
adaptability of algae, they are grouped as halophilic
(salt requiring for optimum growth) and halotolerant (having response mechanism
to survive in saline medium). Generally salinity level between
20-24g/L is the best for algae growth. This adjustment can be done diluting sea
water with tap water. Salinity can be measured using hydrometer. Low
salinity level is changed the biochemical composition of marine microalgae. High salinity has been reported