Abstract The objective of this lab is to determine if changes in pH, enzyme concentration, or if the temperature affected the activity of enzymes. Enzymes “A substance produced by a living organism that acts as a catalyst to bring about a specific biochemical reaction”However, the activity of an enzyme can be also affected by physical factors such as pH, enzyme concentration, and temperature. The reason for this is because these factors change the structure of enzymes. It was hypothesized that the enzyme would denature if any of the factors listed above changedHypothesis: Our hypothesis our group made was that we expected that the enzymes will perform “better” when its exposed to room temperature than when it is exposed to cold or hot temperatures.
But we later found out that the higher the temperature, the better the performance of the enzymes will be but as the temperature reaches a certain high degree, enzymes start to denature or lose function. Introduction: All enzymes are proteins and they have a specific job to do. All cells perform a wide variety of chemical reaction that is vital for a cell’s survival.
However, many of these reactions do not occur and require help in order to proceed. Chemical reactions can be sped up or encouraged to proceed by increasing the temperature or pressure.Objective: To obtain experience with the spectrophotometer and learn the technique of calorimetry. To lean general properties of enzymes. And to learn and conduct an enzymatic experiment. Materials: • Spectrophotometer(s)• Distilled water (DI) water • Parafilm • Test tubes and test tube racks • Pipettes, micropipettes, and tips• Potato extract containing the enzyme Catecholase• Catechol (substrate of Catecholase)• Water bath(s)• Buffer solutions Discussion: Conducting the experiment It appears that the lower the pH, the lower the reaction, Designing our own experimentFor our group experiment, we used four test tubes Test tube (3)- PH 9/5mL Test tube (4) PH7, the Test tube (5)- PH5, Control- Distilled water.
We tested the way PH affects the reachability of Catecholase Catechol. Our hypothesis was that the reaction will be higher at PH-7, our outcome was that PH-7 test tube # 4 produced the best reaction because water is an effective solvent and enzymes work best at PH-Results: Ph Catecholase T3 9 1mL 1mL 0T4 7 1mL 1mL 2.7T5 5 1mL 1mL .
06T1 7 1mL 1mL 0 Error & precaution: Possible Human Error: Improper amount of pH buffer.The solution did not incubate for the proper amount of time.Improper mixing. A solution not heated for long enough.
Iimproper dilution of a solution withdistilled water.An error that our group made was that we didn’t mix the yeast properly therefore causing in a failed experiment. Reference: Lecture Text: Biological Science (5th edition, 2013) by Scott Freeman; Pearson Benjamin Cummings, San Francisco, CA. Website: http://www.master